Fox McCloud appears as a major crossover character from Star Fox, hinting at a shared Nintendo universe.

Team Star Fox including Falco, Peppy, and Slippy appear alongside Fox McCloud.

Pikmin creatures appear in a background scene, referencing the popular Nintendo strategy series.

Princess Daisy makes her debut in the post credit scene, setting up future Mario movies.

R.O.B. appears as a cameo, referencing classic Nintendo hardware and Smash Bros.

Dry Bowser appears as Bowser’s skeletal form during the battle sequence.

Mr Game and Watch appears during combat as a nostalgic 2D character cameo.

Wart from Super Mario Bros 2 appears as a villain in a casino sequence.

Birdo appears alongside Mouser, referencing classic Mario enemies.

Ukiki, the mischievous monkey, steals items and appears again in the ending scene.

Whittle creatures appear in the Galaxy setting as part of the world building.

Honey Queen from Super Mario Galaxy appears as a large supporting character.

Baby Mario and Baby Luigi appear after a transformation sequence.

Donkey Kong returns briefly, connecting the sequel to the original movie.

Ninji enemies appear during action sequences, referencing classic Mario games.